Steady-state photophysical data for ABN in DNA oligonucleotides.

Oligoa	λ abs, max/nm	λ em, max/nm	Φ em	T m/°C	ΔTmb/°C
ODN1	450	530	0.55	63.6 ± 0.6	+3.1
ODN1:ODN2	440	530	0.53	61.8 ± 0.4	−4.7
ODN1:ODN3	468	523	0.40	61.3 ± 0.6	+1.6c
ODN4	452	540	0.49	—	—
ODN4:ODN5	440	525	0.51	39.9 ± 0.3	−0.5
ODN4:ODN6	470	523	0.29	41.3 ± 0.3	−7.3c
ODN7	d	532	0.62	—	—
ODN7:ODN8	d	532	0.50	34.3 ± 0.3	−14.1
ODN7:ODN9	d	532	0.55	37.9 ± 0.3	−11.8c

^aOligonucleotide sequences are given in Fig. 3.

^bΔT_m = T_m for ABN-containing duplex listed in the table row − T_m for the corresponding duplex with canonical thymidine in place of ABN.

^cComparison with T_m for the corresponding natural duplex with a C:G base pair.

^d λ _ex,max is concentration-dependent; see Fig. S18.