Variation of the ASO or SNA based on their base modification, target, spacer region and conjugation. FANA = 2′-fluoroarabino nucleic acid with phosphothioate (PS) linkages. ASO = antisense oligonucleotide with PS linkages against firefly luciferase. SNA = strand with 12 units of dodecanediol that assembles into spherical nucleic acid. PO-dN_4/8 = 4 or 8 nucleotide spacer with phosphate linkages (PO). PS-dN_4/8 = 4 or 8 nucleotide spacer with PS linkages. Survivin = FANA antisense oligonucleotide with PS linkages against survivin. NegativeASO = non-targeting sequence with PS linkages. mmASO = antisense oligonucleotide against firefly luciferase with 3 mismatches and PS linkages. APOB = FANA antisense oligonucleotide against APOB with PS linkages.

Strand name	Base modification	Target	Spacer	Conjugate	Symbol
FANA-ASO	FANA	Luciferase	—	—
FANA-SNA	FANA	Luciferase	—	(Dodecanediol) × 12
FANA-(PO-dN4)-ASO	FANA	Luciferase	(PO-dN4)	—
FANA-(PO-dN4)-SNA	FANA	Luciferase	(PO-dN4)	(Dodecanediol) × 12
FANA-(PS-dN4)-ASO	FANA	Luciferase	(PS-dN4)	—
FANA-(PS-dN4)-SNA	FANA	Luciferase	(PS-dN4)	(Dodecanediol) × 12
FANA-(PO-dN8)-ASO	FANA	Luciferase	(PO-dN8)	—
FANA-(PO-dN8)-SNA	FANA	Luciferase	(PO-dN8)	(Dodecanediol) × 12
FANA-(PS-dN8)-ASO	FANA	Luciferase	(PS-dN8)	—
FANA-(PS-dN8)-SNA	FANA	Luciferase	(PS-dN8)	(Dodecanediol) × 12
FANA-survivin-(PO-dN4)-ASO	FANA	Survivin	(PO-dN4)	—
FANA-survivin-(PO-dN4)-SNA	FANA	Survivin	(PO-dN4)	(Dodecanediol) × 12
PS-DNA-ASO	—	Luciferase	—	—
PS-survivin-ASO	—	Survivin	—	—
PO-DNA-negativeASO(18)	—	—	—	—
PS-DNA-mmASO	—	Mismatch to luciferase	—	—
FANA-(PO-dN4)-mmASO	FANA	Mismatch to luciferase	(PO-dN4)	—
FANA-(PO-dN4)-mmSNA	FANA	Mismatch to luciferase	(PO-dN4)	(Dodecanediol) × 12
FANA-DNA-NegativeASO	FANA	—	—	—
FANA-DNA-NegativeSNA	FANA	—	—	(Dodecanediol) × 12
PS-(PO-dN4)-DNA-SNA	—	Luciferase	—	(Dodecanediol) × 12
FANA-APOB-(PO-dN4)-ASO	FANA	APOB	(PO-dN4)	—
FANA-APOB-(PO-dN4)-SNA	FANA	APOB	(PO-dN4)	(Dodecanediol) × 12
Control	—	—	—	—	No strand was used