Fig. 1. Formation of Gly oligomers under wet–dry cycles and the effect of POPC on their overall yield as analysed by LC-MS. (A) Upon five wet–dry cycles at 90 °C, 80 mM Gly solution shows the formation of Gly oligomers of up to 6-mers. (B) Relative abundances of individual oligomers are summed up to give the overall oligomer yield, which was found to be lower in the presence of POPC (Gly + POPC) than that of the Gly control (Gly − POPC) reaction. All glycine peptides were detected and quantified using LC-MS (see Methods for more details). The error bars represent standard deviation (N = 4).
