Fig. 10. (a) Schematic illustration of imaging of APN for detection of bladder cancer using CyP1 (R = H or CH₂CHOHCH₃). (b) Fluorescence spectra of CyP1 and CyP2 (30 μM) in the absence or presence of APN (0.5 mg mL⁻¹) in PBS (10 mM, pH 7.4). (c and d) RCE as a function of time post-injection of CyP1 and CyP2 in living mice. The three lines represent the measurements in three independent mice. (e) Representative NIRF images of living mice at t = 0 and 150 min post-injection of CyP1 at different time points post tumor implantation. NIRF images acquired at 720 nm upon excitation at 675 nm. (f) NIRF intensities of the bladder in living mice after 150 min injection of CyP1 at the different time points post tumor implantation. PBS versus tumor implantation groups, *p < 0.05, **p < 0.01, ***p < 0.001. Reproduced with permission from ref. 75. Copyright 2020 John Wiley and Sons.