Fig. 8. Bioimaging of the (a–d) crude red emissive CNDs obtained from OPDA and (e–h) the column purified red component. All the three color images (blue, green and red) were observed in all the three channels (blue, green and red) for the crude material, while only red color was observed for the red component. (i) Cytotoxicity data of the crude and all three column separated components, suggesting the non-toxic nature of the materials. (j & k) Fluorescence lifetime imaging and the corresponding distribution. The data show a homogeneous lifetime of the red component throughout the cell with a FWHM of 0.4 ns.
