Fig. 2. Inhibition of Aβ-42 fibril formation in the presence of the condensates (A) time evolution of the ThT fluorescence intensity of samples incubated in glass bottom plates for 3 days: 4 μM Aβ-42 solution without (violet) and with 20 μM Laf1-AK-Laf1 (orange). The dispersed phase was re-suspended by mechanical agitation before analysis. (B) Residual Aβ-42 monomer measured by size exclusion chromatography. Values correspond to the areas under the monomer peak in the chromatograms measured for freshly purified monomeric 4 μM Aβ-42 solution before incubation (“Before”) and after incubation without (−LL) and with (+LL) 20 μM Laf1-AK-Laf1 (“After”). (C) and (D) Representative TEM images of fibrils formed in homogeneous solutions of 4 μM Aβ-42 in the absence of Laf1-AK-Laf1 after 3 days (C), and of a solution of 4 μM Aβ-42 incubated with 20 μM Laf1-AK-Laf1 for 3 days, showing lack of fibrils (D).