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. 2021 Feb 8;12(13):4810–4818. doi: 10.1039/d0sc06744j

Fig. 5. (A) Amplified CRET-induced detection of miRNA-155 by coupling the HCR generating hemin/G-quadruplex polymers to the Fl-loaded NMOFs. (B) Chemiluminescence and CRET-generated fluorescence of Fl upon analyzing various concentrations of miRNA-155 by the scheme outlined in (A): (a) 0 nM; (b) 0.5 nM; (c) 10 nM; (d) 25 nM; (e) 200 nM. (C) Calibration curve corresponding to the CRET-generated fluorescence of Fl in the presence of variable concentrations of miRNA-155: (a) using the HCR-amplified CRET-detection scheme shown in (A); (b) using the non-amplified hairpin-functionalized NMOFs according to Fig. 4(A). (D) Chemiluminescence and CRET-generated fluorescence of Rh 6G upon analyzing various concentrations of miRNA-21 according to the scheme in Fig. S8:† (a) 0 nM; (b) 5 nM; (c) 50 nM; (d) 100 nM; (e) 333 nM. (E) Calibration curve corresponding to the CRET-generated fluorescence of Rh 6G in the presence of variable concentrations of miRNA-21: (a) using the HCR-amplified CRET-detection scheme shown in Fig. S8;† (b) using the non-amplified hairpin-functionalized NMOFs according to Fig. 4(E). Error bars derived from N = 3 experiments.

Fig. 5