Fig. 6. Multiplexed analysis of miRNA-155 and miRNA-21 by a mixture consisting of the Fl-loaded hp5-modified NMOFs and the Rh 6G-loaded hp6-modified NMOFs using the HCR-amplified CRET-induced analysis of the two miRNAs upon (panel A) subjecting the mixture to variable concentrations of miRNA-155: (a) 0 nM; (b) 1 nM; (c) 20 nM; (d) 50 nM; (e) 250 nM; (panel B) subjecting the mixture to variable concentrations of BRCA1: (a) 0 nM; (b) 5 nM; (c) 50 nM; (d) 100 nM; (e) 333 nM; and (panel C) subjecting the mixture to variable concentrations of miRNA-155: (a) 0 nM; (b) 1 nM; (c) 10 nM; (d) 50 nM; (e) 200 nM (curve (b) in panel II), and to variable concentrations of miRNA-21: (a) 0 nM; (b) 5 nM; (c) 50 nM; (d) 100 nM; (e) 500 nM (curve (a) in panel II). Error bars derived from N = 3 experiments.