Fig. 7. (A) Fl-loaded hairpin hp7-functionalized UiO-66 NMOFs for the CRET analysis of gene p53. The p53-triggered opening of the hairpin leads, in the presence of K+-ions and hemin, to the self-assembly of the hemin/G-quadruplex that catalyzes the generation of chemiluminescence, λem = 420 nm, and the CRET-induced fluorescence of Fl, λem = 520 nm. (B) CRET-induced fluorescence spectra of Fl, λem = 520 nm, in the presence of variable concentrations of p53: (a) 0 nM; (b) 10 nM; (c) 25 nM; (d) 100 nM; (e) 200 nM. (C) Derived calibration curve corresponding to the CRET fluorescence signals of Fl generated at variable concentrations of p53. Error bars derived from N = 3 experiments.
