Fig. 6. Applications of BCP nanoparticles prepared by living CDSA in nanomedicine. (A) Schematic illustration of DNA (blue) complexation and subsequent collapse at the quaternized P2VP corona (purple) of a water-soluble cylindrical micelle. TEM images of the quaternized P2VP micelles in iPrOH (1) and DNA complexed micelles after dialysis into water (2). (B) Overlaid laser scanning confocal microscopy images of cells incubated with polyfluorene fiber-like micelles functionalized with tracking (BODIPY dye) and targeting (folic acid) groups (4). Nuclei stained blue (1), F-actin stained green (2) and micelle emission red (3). TEM image of fiber-like micelle uptake (5) showing end-on interactions with the cell membrane (circled in blue) and micelle fragments (circled in yellow). (C) Platelet micelles with PLLA cores used as adhesives for thick discs of calcium-alginate hydrogels. The hydrogel discs demonstrated enhanced resistance to breaking under strain (discs coloured with different dyes) (1). The adhesive energy from bulk shear for these hydrogels far exceeded those prepared using spherical and cylindrical morphologies or adhered with water as a control (2). Panel (A) reproduced from ref. 250. Panel (B) reproduced from ref. 251. Panel (C) reproduced from ref. 252.