Fig. 2. 1H NMR spectrum (400 MHz, D2O) of (a) PAM (5 mM), (b) PAM (5 mM) + CP6A (5 mM) and (c) CP6A (5 mM). (Inset) Partially enlarged 1H NMR spectrum of the host–guest complex. (d) Fluorescence titration of CP6A (1.00 μM) with PAM in 10 mM PBS buffer at pH 7.4, λex = 290 nm. (Inset) The associated titration curve at λem = 330 and fit according to a 1 : 1 binding stoichiometry. Data were from n = 3 independent experiments and are presented as mean ± SD.
