Figure 2.

Expression of various semaphorins are elevated in Olig2⁺ cells after SCI. (A–E) Expression of Sema3d (A), 4c (B), 4d (C), 5a (D), and 6d (E) mRNA in Olig2⁺ cells (green) in the thoracic spinal cord at 1 week post-injury. Sema mRNAs were detected by in situ hybridization and Olig2 proteins were visualized by immunohistochemistry. Arrowheads indicate Sema⁺/Olig2⁺ cells. Scale bar, 50 μm. (F, G) Percentages of different cell types (Olig2⁺, NeuN⁺, Sox9⁺, and Iba1⁺ cells) in Sema3d⁺, 4c⁺, 4d⁺, 5a⁺, 6a⁺, and 6d⁺ cells (F) and percentages of Sema3d⁺, 4c⁺, 4d⁺, 5a⁺, 6a⁺, and 6d⁺ cells in each cell type (G).