Fig. 1. (A) Mass spectrum of amylin (10 μM) in 20 mM ammonium acetate. Each peak is labeled by its nominal n/z ratio. The bottom spectrum exhibits oligomeric species with a relatively low intensity that is not easily identifiable in the top spectrum. The inset shows the isotopic spacing of n/z = 1/3 which exhibits the presence of a dimer with z = +6. (B) The ATD of amylin at m/z = 976. (C) Mass spectrum of CGRP (10 μM) in 20 mM ammonium acetate. The insrt shows the isotopic spacing of n/z = 1/3 which indicates that this mass spectral peak correlates to a triply charged dimer. (D) The ATD of CGRP at m/z = 948 and a REMD conformation of CGRP monomer with the segment 8–18 colored in red. This conformation is consistent with previous NMR and CD studies of CGRP in structure-inducing solvent.^70,72,73.