Fig. 4.

Pharmacologic inhibition of Axl inhibits osteoclast formation. Osteoclast progenitor cells were seeded in a 24-well plate (2 × 10⁴ cells/well), treated with or without 35 ng/ml RANK-L and varying concentrations of BGB324. Five days later, mature and differentiated osteoclasts were fixed and stained for tartrate resistant acid phosphatase (TRAP). a Representative images of TRAP-positive, multinucleated osteoclasts, 5X magnification. b Total number of TRAP-positive, multinucleated ($\ge$3 nuclei) osteoclasts, analyzed by One-way ANOVA. c Scheme of isolating primary osteoclast precursor cells from 6–8 week-old female BALB/c mice. d Representative images of TRAP-positive, multinucleated osteoclasts differentiated from primary osteoclast precursor cells of BALB/c mice, 5X magnification. e Total number of TRAP-positive, multinucleated ($\ge$3 nuclei) osteoclasts differentiated from primary osteoclast precursor cells, analyzed by Student’s t-test. Results are the mean and standard error values of three independent experiments. ***p < 0.0001, ****p < 0.00001