Fig. 6.

MCP-1 is a downstream effector of Axl in osteoclast precursor cells. Conditioned media of shSCM and shAXL osteoclast progenitor cells were collected and analyzed by cytokine array (a, b). c MCP-1 expression in shSCM and shAXL osteoclast progenitor cells were assessed by qPCR. Osteoclast progenitor cells were seeded and treated with or without 35 ng/ml RANK-L and/or 50 ng/ml MCP-1. Five days later, mature and differentiated osteoclasts were fixed and stained for tartrate resistant acid phosphatase (TRAP). d Representative images of TRAP-positive, multinucleated osteoclasts, 5X magnification. e Total number of TRAP-positive, multinucleated ($\ge$3 nuclei) osteoclasts were quantified. *p < 0.05, **p < 0.01, ***p < 0.0001