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. 2021 Jun 5;7:135. doi: 10.1038/s41420-021-00520-1

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — A Western blot analyses for endogenous expression of PGK1 in various lung adenocarcinoma cells. Tubulin was used as the internal control for protein loading. B Representative Giemsa staining of several lung adenocarcinoma cells including H23, CL1-0, H441, and CL1-5, after migration through a Boyden’s chamber. Scale bar: 100 μm. C Quantification of the migration ability of the lung adenocarcinoma cells panel. D The correlation of PGK1 expression and the metastatic lung cancer cell lines was analyzed by the nonparametric Spearman method. E The determination of PGK1 and PGK2 protein levels in CL1-0 cells with vector control or with PGK1 overexpression. The determination of PGK1 protein levels in CL1-5 cells after transfection with nonsilencing and PGK1 shRNAs. F Migration ability of the PGK1-overexpressing stable clone of CL1-0 cells was determined compared to the vector control stable clone (upper). Migration ability of the PGK1 knockdown stable clones of CL1-5 cells was determined, compared to the nonsilencing shRNA control stable clone (lower). Scale bar: 100 μm. G Quantitation the migration ability of the PGK1 knockdown models. The migration activities of the CL1-5 cells transfected with the designated shRNA clones of PGK1 (bottom). The data from three independent experiments are shown as the mean ± SD. The symbol ***p < 0.001 in the nonparametric Mann–Whitney test.