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. 2021 Jun 5;7:135. doi: 10.1038/s41420-021-00520-1

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — A The molecules that indicated to interact in the PGK1- or PGK2-based proteomics profiles according to BioGrid website analysis. B Western blot analysis of PGK1 and HTATSF1 expression from CL1-0 and H1355 cells with the forced expression of the vector control or exogenous PGK1 gene. C Western blot analysis of PGK1 and HTATSF1 protein levels in the nuclear protein fraction and cytoplasmic fraction derived from PGK1 two-way models. D Pull-down assay for whole-cell lysates derived from CL1-0 and H1355 cells with the forced expression of PGK1 or vector control using beads followed by western blot analysis of the HTATSF1 and PGK1 proteins. E Western blot analysis of HTATSF1, PGK1 and PGK2 expression from CL1-0 cells with or without the shHTATSF1 clones. F Pull-down assay for whole cell lysates derived from CL1-0 PGK1 cells with or without the shHTATSF1 clones using beads followed by western blot analysis of HTATSF1 proteins. G Representative Giemsa staining to estimate the migration abilities of the H1355 PGK1 cells transfected with the designated shRNA clones of the HTATSF1 gene. Scale bar: 100 μm. H Cellular migration abilities of the H1355 PGK1 cells transfected with the designated shRNA clones of the HTATSF1 gene. The symbols **p < 0.01 and ***p < 0.001 in the nonparametric Mann–Whitney test.