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. 2021 Jun 5;12(6):581. doi: 10.1038/s41419-021-03871-w

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — A qRT-PCR demonstrating the expression of c-Myc, HuR, and Pirh2 mRNA in Pirh2 knockdown (Pirh2 KD), HuR knockdown (HuR KD), and control (scrambled) H1299 cells used for RNA immunoprecipitation (RIP assay). B The scheme of c-Myc-coding mRNA (NCBI Reference Sequence: NM_002467.6) with indicated regions covered by primes used for RIP assay. CDS, coding sequence; UTR, untranslated regions. C RNA immunoprecipitation results obtained by qRT-PCR.