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. Author manuscript; available in PMC: 2022 Jun 3.
Published in final edited form as: Mol Cell. 2021 Apr 20;81(11):2428–2444.e6. doi: 10.1016/j.molcel.2021.03.044

Figure 3. The FANCM-FA core complex interaction specifically mediates Tus/Ter-induced STGC.

Figure 3.

See also Figure S3. A. FancmΔMM1 allele and RT qPCR analysis of MM1 and MM2 encoding mRNA in Fancm+/− and FancmΔMM1/− cells. Red half-arrows: RT qPCR primers. Here and in all subsequent expression analyses, data normalized to Gapdh mRNA using the 2−ΔCT method (see STAR methods). Data shows mean ± SD. Analysis by Student’s t-test (n=3). B. Immunoblot of chromatin-extracted FANCM in Fancm+/− and FancmΔMM1/− clones. *: non-specific band. C. Immunoblot showing FANCD2 ubiquitination in Fancm+/− and FancmΔMM1/− clones. D. Proliferative competition assay in MMC, measuring enrichment of GFP+ Fancm+/− vs. GFP FancmΔMM1/− cells. Data shows mean ± SD (n=3). E and F. ChIP analysis of FANCM (E) and FANCA, FANCL and BLM (F) at Tus/Ter in Fancm+/− and FancmΔMM1/− cells. Data shows mean ± SD. Analysis by one-way ANOVA (n=3). G. Tus/Ter-induced repair in Fancm+/− (white) clones vs. FancmΔMM1/− (gray) clones. Data shows mean ± SEM. Analysis by one-way ANOVA (n=5). H. Tus/Ter-induced repair in Fancm+/− vs. FancmΔMM1/− clones co-transfected with Tus and siRNAs as shown. Data shows mean ± SEM. Analysis by Student’s t-test (n=6).