KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Rabbit Anti-HA tag antibody | Abcam | Cat#ab9110, RRID:AB_307019 |
| Rabbit anti-BLM Antibody | Bethyl labs | Cat#A300-110A, RRID:AB_2064794 |
| Rabbit anti-FANCL antibody | Abcam | Cat#ab94458, RRID:AB_10675676 |
| Rabbit anti-FANCM antibody | Abcam | Cat#ab95014, RRID:AB_10675719 |
| Rabbit anti-Histone H3 antibody | Abcam | Cat#ab1791, RRID:AB_302613 |
| Rabbit anti-FANCA antibody | Abcam | Cat#ab97578, RRID:AB_10680297 |
| Rabbit anti-FANCD2 [EPR2302] antibody | Abcam | Cat#ab108928, RRID:AB_10862535 |
| mouse anti-BrdU antibody | Abcam | Cat#ab8039 RRID:AB_306213 |
| Goat anti-mouse FITC conjugated IgG | Jackson Immuno Research | Cat#115-095-146 RRID:AB_2338599 |
| Bacterial and Virus Strains | ||
| One Shot Stbl3 Chemically Competent E. coli | ThermoFisher Scientific | Cat#C737303 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| 5-adamantyl-indoleacetic acid | TCI Chemicals | Cat#A3390 |
| Asunaprevir | MedChem Express | Cat#BMS-650032 |
| Spy NLS Cas9 | New England Biolabs | Cat#M0646T |
| 5mL 4mg/mL RNaseA | Qiagen | Cat#158924 |
| proteinase K | Fisher Scientific | Cat#BP1700500 |
| sodium bicarbonate | Fisher Scientific | Cat#S233500 |
| formaldehyde, ACS reagent, 37% | Sigma-Aldrich | Cat#252549-25ML |
| Complete Protease Inhibitor Cocktail Tablets | Sigma-Aldrich | Cat#4693132001 |
| Mitomycin C from Streptomyces caespitosus | Millipore Sigma | Cat#475820-10MG |
| Lipofectamine 2000 | ThermoFisher Scientific | Cat#11668019 |
| Novex TBE-Urea gels, 10%, 10 well | ThermoFisher Scientific | EC6875BOX |
| Bolt 4-12% Bis-Tris 1 mm Mini Protein Gel | ThermoFisher Scientific | NW04122BOX |
| Magna ChIP Protein A+G magnetic beads | Fisher Scientific | Cat#16-663 |
| UltraPure Salmon Sperm DNA Solution | ThermoFisher Scientific | Cat#15632011 |
| 10mg/mL BSA, Molecular Grade | New England Biolabs | Cat#B9000S |
| Propidium Iodide | Sigma-Aldrich | P4170-100MG |
| Critical Commercial Assays | ||
| EnGen®sgRNA Synthesis Kit, S. pyogenes | New England Biolabs | Cat#E3322S |
| PCR Purification Kit | Qiagen | Cat#28106 |
| RNeasy Mini Kit | Qiagen | Cat#74106 |
| Endo-free Maxiprep kit | Qiagen | Cat#12362 |
| 2x Power SYBR Green | Applied Biosystems | Cat#4368702 |
| Power SYBR Green RNA-to CT 1-Step Kit | Applied Biosystems | Cat#4389986 |
| Taq DNA Polymerase Kit (250 U) | Qiagen | Cat#201203 |
| MycoAlert Mycoplasma Detection Kit | Lonza | Cat#LT07-318 |
| RNA Clean and Concentrator Kit | Zymo Research | Cat#R1017 |
| Cell line | ||
| Brca1 exon 11 conditional mouse ES cells | Dr. Chuxia Deng (Xu et al., 1999) | N/A |
| Oligonucleotides | ||
| See Supplemental Table S1. | ||
| Recombinant DNA | ||
| Plasmid: pHIV-Tir1-74A-8xMYC-IRES-NAT-T2A-hCD52 | This study and (Willis et al., 2018) | N/A |
| Plasmid: pcDNA-1xAID-8xHA-SMASH-3xHA-T2A-neo (mBlm C-term targeting) | This study | N/A |
| Plasmid: pcDNA3β-MYC-NLS-Tus-F140A-3xHA | This study | N/A |
| Plasmid: pX330-U6-Chimeric_BB-CBh-hSpCas9(1.1) | This study | Addgene #71814 |
| Plasmid: pcDNA3β -myc NLS-I-SceI | (Puget et al., 2005) | N/A |
| Plasmid: pcDNA3β | (Puget et al., 2005) | N/A |
| Plasmid: pcDNA3β -myc NLS-Tus | (Willis et al., 2014) | N/A |
| Software and Algorithms | ||
| Prism 7.0e for Mac | GraphPad Software | https://www.graphpad.com/scientific-software/prism/, RRID:SCR_000306 |
| CCTop-CRISPR/Cas9 target gene predictor | (Stemmer et al., 2015) | https://cctop.cos.uni-heidelberg.de, RRID:SCR_016890 |
| Other | ||
| Diagenode Bioruptor Plus UCD-300 TM | Diagenode | Cat#B01020001 |
| Eppendorf Thermomixer C (REF 5382000023) | Fisher Scientific | Cat#05-412-503 |
| Smartblock Thermoblock (REF 5367000025) 24x 1.5-2.0mL | Fisher Scientific | Cat#05-412-510 |
| Diagenode Bioruptor water cooler | Diagenode | Cat#B02010003 |
| Medmark Technologies Nutator mixer | Fisher Scientific | Cat#NC0597936 |
| Dynal MPC-S Magnetic Rack #A13346 | Fisher Scientific | Cat#501148229 |
| Deposited data | ||
| Unprocessed blots and gels | This paper | https://data.mendeley.com/datasets/gwis4kp9mg |
| Whole genome sequencing data | This paper | SRA BioProject ID: PRJNA704440 |