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. Author manuscript; available in PMC: 2022 May 12.
Published in final edited form as: Nano Lett. 2021 May 3;21(9):4078–4085. doi: 10.1021/acs.nanolett.1c01029

Figure 3.

Figure 3.

Phosphotyrosine is critical for rapid cellular uptake. (A) Confocal fluorescence images of HEK293_TNAP-RFP cells incubated with NBD-1p or NBD-1 (200 μM, 12 h, washed), respectively. (B) ImageJ analysis of intracellular fluorescence intensity of HEK293_TNAP-RFP cells in (A). Data is presented as mean ± standard deviation, *** means p<0.001. (C) Confocal fluorescence images of HEK293_TNAP-RFP cells incubated with NBD-(D)Sp (400 μM, 12 h, washed). (D) Dephosphorylation rate of NBD-1p and NBD-(D)Sp by ALP (0.1 U/mL, 37 °C).