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. 2021 Jun 5;12:329. doi: 10.1186/s13287-021-02414-6

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — Bu/Cy preconditioning changes bone marrow cellularity and upregulates Col4a1 and Col4a2 expression in bone marrow. a Schematic representation of the experimental timeline. BALB/c mice were divided into two groups: untreated control and Bu/Cy (n = 5/group). The Bu/Cy group received 20 mg/kg/day Bu for 4 days and 100 mg/kg/day Cy for 2 consecutive days. After 48 h, mice were sacrificed and used for further analysis. b Slides of mouse femur sections were stained with hematoxylin and eosin (H&E). Cellularity was defined as the ratio of the hematoxylin-stained area within the medullary cavity of the diaphysis, corresponding to the area occupied by BMCs, to the selected total area. Scare bar 200 μm. c The RT2 profiler PCR array (for the analysis of mouse ECM and adhesion molecule mRNA expression) was used for real-time quantitative PCR (RT-qPCR) (n = 3/group). Data were analyzed using the Excel sheet provided by the manufacturer. Gusb and β-actin were selected as reference genes. d The mRNA expression levels of Col4a1 (left) and Col4a2 (right) were verified by RT-qPCR using custom oligos. The data represent the mean ± S.E.M. and were analyzed using the Student’s t test (*P < 0.05)