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. 2021 Jun 5;12:329. doi: 10.1186/s13287-021-02414-6

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — MMP3 expression and secretion is increased in TMSCs compared to other MSCs. a Nano-liquid chromatography with tandem mass spectrometry analysis was performed using TMSC-CM, BMMSC-CM, and ADMSC-CM. Peptides of ECM-modulating enzymes are listed. Detected peptides are depicted in black. b The mRNA expression levels of MMP3, MMP1, CTSB, and PEPD in human TMSCs, BMMSCs, and ADMSCs were determined using real-time quantitative PCR (RT-qPCR) with custom oligos. GAPDH was used as the reference gene. c MMP3 protein expression levels in MSC lysates were determined by quantitative western blot analysis by dividing the pixel densities of the MMP3 bands by the pixel densities of the corresponding β-actin bands. d MMP3 protein concentrations in MSC-CM were quantified using a human MMP3 ELISA kit. The data represent the mean ± S.E.M. and were analyzed using one-way ANOVA (*P < 0.05)