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FGF signalling perturbation using the SU5402 inhibitor in brittle star embryos. (A) Experimental procedure for SU5402 treatment. (B) Phenotypic analysis of SU5402-treated A. filiformis embryos and controls at 58 hpf and 4 days post fertilization shows that perturbation of FGF signalling results in embryos with no skeletal spicules forming. Numbers at the bottom show counts for embryos observed with the represented phenotype/total embryos counted. Scale bars: 50 μm.
