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. 2021 May 27;148(10):dev180760. doi: 10.1242/dev.180760

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© 2021. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 3. — FGF signalling perturbation using SU5402 in brittle star regenerating arm explants. (A) Experimental procedure for SU5402 treatment. (B) Phenotypic analysis of SU5402-treated A. filiformis regenerates and controls at 24 h post treatment (stage 3) shows that perturbation of FGF signalling inhibits spicule formation. Insets show detail of spicules. Numbers at the bottom show counts for explants observed with the represented phenotype/total explants counted. Red line, amputation plane. Dashed lines, outline of regenerating bud. Scale bars: 50 μm.