Fig. 1.

Expression of BKα in RAW264.7 macrophages. a Representative Western blots of BK channel and lamin B1 expression in RAW 264.7 macrophages; whole cell (WC); nuclei (N); nuclei membrane (NM); membrane denuded nuclei preparations (MDN) preparations. Gels loaded for protein 20ug or initial cell number 100,000 equivalents. b Representative blots of lamin B1, COX IV, GAPDH and calnexin in whole cell and nuclei preparations c Representative immunofluorescence of whole cells for BK channel. (i) DAPI only; (ii) BK channel antibody; (iii) Merged DAPI + BK channel antibody. Scale bar 5μM