Fig. 3.

Blockade of nuclear BK channels induces CREB phosphorylation in isolated nuclei. Western blot of pCREB and CREB expression in nuclei, stimulated with indicated solution for 5 min. a Representative Western blot from one experiment. 10ug protein was loaded into each lane. b Densitometry analysis of immunoblots. The results are 3 independent experiments. Kruskal–Wallis one-way analysis of variance, followed by Dunn’s post hoc test. Error bars represent median and interquartile ranges. NT vs. 200 nM Ca²⁺ *p = 0.0168. DMSO vs. 100 nM paxilline *p = 0.0168. NT no treatment control, DMSO 0.01% DMSO