Fig. 4.

Ca²⁺ and calmodulin-dependent kinase inhibitors reduce paxilline-induced CREB phosphorylation in whole cells. Western blot of pCREB and CREB expression following pre-treatment of whole cells with 5 μM STO609, 5 μM tatCN21, 5 μM tats or 100 mM NaOH for 10 min and subsequent stimulation with 1 μM paxilline or DMSO for 25 min. a Data representative of one repeat. Approximate molecular weight of the protein of interest is indicated in kDa. 10 ug protein was loaded into each lane. b, c Densitometry analysis of immunoblots. The results are representative of at least 4 independent experiments. Kruskal–Wallis one-way analysis of variance, followed by Dunn’s post hoc test. Error bars represent median and interquartile ranges. DMSO vs. paxilline **p = 0.0023, DMSO vs. paxilline + tats *p = 0.0133. p phosphor, CREB cyclic AMP response element binding protein, DMSO 0.01% dimethyl sulfoxide control, tats scrambled tatCN21 control