Fig. 2.

Cytotoxicity evaluation of STS in MCF-7 and HMEC. a MCF-7 cells were exposed to STS at various concentrations for 48 h, and then cell viability was detected by MTS assay. Data are expressed as mean ± SD (n = 4). Statistical significance from the one-way ANOVA, Tukey’s post hoc test: **p < 0.01, or ***p < 0.001 vs. control group. b. HMEC cells were exposed to STS at various concentrations for 48 h, and then cell viability was detected by MTS assay. Data are presented as mean ± SD (n = 4). Statistical significance from the one-way ANOVA, Tukey’s post hoc test: *p < 0.05, or ***p < 0.001 vs. control group. c Under phase contrast microscopy, morphological changes MCF-7 cells were observed with 12 and 24 µM of STS for 48 h. d MCF-7 cells treated with 1.2, 12, and 120 µM of sodium thiosulfate for 48 h. Data are expressed as mean ± SD (n = 4). Statistical significance from the one-way ANOVA, Tukey’s post hoc test: *p < 0.05, **p < 0.01, or ***p < 0.001 vs. control group