Fig. 3.
Effects of STS on cell cycle in MCF-7 cells. a Representative histogram of MCF-7 cells treated with STS (0, 12 and 24 µM) for 24 h. b Representative immunocytochemical images with PCNA antibody of MCF-7 cells after treatment with STS 24 µM for 48 h. c Quantification of nuclear expression of PCNA by ImageJ software. The images of 50 cells of each group were analyzed. Data are expressed as mean ± SD (n = 50 per group). Statistical significance from Student’s t-test: ***p < 0.001