Fig. 6.

Effects of STS on ROS and GSH levels in MCF-7 and HMEC derived from human mammary epithelial cells. a MCF-7 cells were exposed to 12 µM STS for 30 min, or tBHP50 µM as a positive control. ROS levels were detected by flow cytometry-based assay using H2DCFDA. Data are presented as mean ± SD. (control: n = 9, STS: n = 4, tBHP: n = 3). Statistical significance from one-way ANOVA, Dunnett’s test: ***p < 0.001 vs. control group. b HMEC cells were exposed to 12 µM STS for 30 min or 50 µM tBHP as a positive control. ROS levels were detected by a flow cytometry-based assay using H2DCFDA. Data are presented as mean ± SD. (control: n = 10, STS: n = 6, tBHP: n = 3). Statistical significance from one-way ANOVA, Tukey’s pot hoc test: ***p < 0.001. c MCF-7 cells were pretreated with NAC for 1 h and exposed to 12 µM STS for 30 min. ROS levels were detected by a flow cytometry-based assay using H2DCFDA. Data are expressed as mean ± SD (n = 5 per group). Statistical significance from two-way ANOVA, Tukey’s post hoc test: ***p < 0.001. d MCF-7 cells were exposed to 12 µM STS for 48 h. GSH levels were measured by luminescence assay. Data are expressed as mean ± SD (n = 4 per group). Statistical significance from Student’s t-test: ***p < 0.001