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. Author manuscript; available in PMC: 2021 Jun 7.
Published in final edited form as: Insect Biochem Mol Biol. 2021 Feb 4;133:103548. doi: 10.1016/j.ibmb.2021.103548

Fig. 4.

Fig. 4.

The interaction of cold and exercise treatments. sestrin and spargel mutants and background controls were housed at 4 °C for 48 h and exercised until fatigued following 14 h of recovery at 25 °C. (A) Cold treatment did not affect endurance in either sestrin mutants or controls. Mutants had significantly lower endurance than controls whether pre-treated or not (n = 8 vials of 10–20 flies each group; p = 0.0002; log-rank test). (B) Cold treatment did not significantly affect endurance in spargel mutants, but mutants had significantly lower endurance whether pre-treated or not (n ≥ 6 vials of 20 flies each group; p = 0.006; log-rank test). sestrin and spargel mutants were exercised for 2 h and immediately transferred to an ice bath for 2 h and CCRT was assessed. Acute exercise did not affect CCRT in either (C) sestrin or (D) spargel mutants. In both cases, mutants had longer CCRT than controls, whether pre-treated or not.