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. 2021 May 24;8:651600. doi: 10.3389/fmed.2021.651600

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Copyright © 2021 Xu, Song, Li, Huang, Liu, Bao and Shen.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Knockdown of EBLN3P suppressed the proliferation of SW480 and HCT116 cells. (A) RT-PCR for the expression of EBLN3P in SW480 and HCT116 cells transfected with sh-EBLN3P-1, EBLN3P-2, or sh-NC. (B) CCK-8 assays determined the cell ability in SW480 and HCT116 cells transfected with sh-EBLN3P-1, EBLN3P-2 or sh-NC. (C) Clone formation capacity of SW480 and HCT116 cells was assessed by the clone formation assays. (D) Apoptotic rate of CRC cells was shown after the transfection. (E) Western blot for the expression of cleaved caspase-3, caspase-3, cleaved caspase-9, and caspase-9 in SW480 and HCT116 cells transfected with sh-EBLN3P-1, EBLN3P-2, or sh-NC. *p < 0.05; **p < 0.01.