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. 2021 May 24;8:651600. doi: 10.3389/fmed.2021.651600

Figure 6.

Figure 6

UHMK1 expression was a potential target of miR-323a-3p. (A) Sequence alignment of human miR-323-3p with 3′-UTR of UHMK1 mRNA predicted by TargetScan. (B) UHMK1 expression in 275 CRC specimens and 349 non-tumor specimens according to the data of TCGA datasets. (C) The correlation between UHMK1 and miR-323-3p expression analyzed in 450 CRC samples. (D) The correlation between UHMK1 and EBLN3P expression analyzed in 471 CRC samples. (E) Decreased UHMK1 mRNA expression was observed in four CRC cells compared to NCM460 cells. (F) The luciferase activity of the wild-type UHMK1 3′-UTR (Wt) and mutant UHMK1 3′-UTR (Mut) cotransfected with miR-323-3p mimics or NC mimics was measured. (G) The levels of EBLN3P and UHMK1 in CRC cells transfected with miR-323-3p mimics or NC mimics by RT-PCR. (H) The levels of EBLN3P and UHMK1 in CRC cells transfected with miR-323-3p mimics or NC inhibitors by RT-PCR. (I,J) Colony formation assays and Transwell assays for the functional exploration of UHMK1 knockdown. **p < 0.01.