Skip to main content
. 2021 Apr 23;29(5):446–455. doi: 10.1016/j.jsps.2021.04.007

Fig. 1.

Fig. 1

Characterization of NL-miR-1296. (A) Particle size, PDI, zeta potential and EE% characterization of NL-miR-1296. (B) Transmission electron microscopy of plain NL, and NL-miR-1296 (C) TEM images. (D) present scanning electron microscope overview image of prepared NL-miR-1296; (E) 1% agarose gel used to test the complexation of miR-1296 with NL at different n/p ratios. Different n/p ratios used 0.5–10, where 0 represent free miR-1296 only. All wells contain the same amount of miR-1296 (0.65 μg) but various NL part. The same n/p ratios were used but with NL were permeabilized with 0.5% triton. NL-miR-1296 were allowed 30 min to complex before running. Run was for 40 min at 80 V using TBE buffer. Ethidium bromide (0.5 μg/mL) was used to stain the gel; (F) Characterization of fresh NL-miR-1296 with different n/p ratios. Data represent mean ± SEM; (G) In vitro release of miR-1296 from NL-miR-1296. The release was evaluated in pH 7.4 using TE buffer, at 37 °C. Free miR-1296 was tested for integrity purposes. Points represent averages ± SEM.