Fig. 4.

Effect of serum, temperature and time on NL-miR-231 stability. Serum stability was tested using 1% agarose gel. (A) simply samples of NL-miR-1296 (n/p ratio of 3) or free miR-1296 was incubated in 1:1 v/v of FBS (final concentration 50%) at 37 °C for 1, 3, and 24 h. (B) samples of the same NL-miR-1296 incubated in FBS for different periods were treated with 0.5%TritonX-100 just before the run and presented next to free miR incubated at the same time and temperature without FBS (denoted by §).