Fig. 5.

Effect of storage time and temperature on physical and biological activity of NL-miR-1296. (A) Interplay between stability and biological activity of NL-miR-1296 on TNBC at different time and storage temperatures. MDA-MB-231 cells were seeded in 24-well plate for 24 h. After 40 or 80 days, cells were transfected with NL-miR-1296 stored at different temperatures. Media was changed after 5 h, and viability was tested using Alamar Blue after 48 h and compared to untreated cells. Freshly prepared NL-miR-1296 was used transfect the cells as described above to represent 0-time point. Physical characterization of NL-miR-1296 stored in different temperatures at different time points was presented as (B) particle size, (C) PDI, and (D) zeta potential. Data represent the mean ± SEM of two independent studies (n = 6). (Multiple student t-test).