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. 2021 May 24;8:675842. doi: 10.3389/fmed.2021.675842

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Copyright © 2021 Zhang, Guo, Yang and Li.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Validation of the biological structure of circ-CYP24A1. (A) Sanger sequencing for the circ-CYP24A1 PCR products. (B) RT-qPCR for the expression of circ-CYP24A1 in A431 and SCL-1 cells under RNase R (-) or RNase R (+). (C,D) RT-qPCR confirming the expression of circ-CYP24A1 in A431 and SCL-1 cells incubated with si-circ-CYP24A1-transfected exosomes. (E) NTA for detecting the particle size of transfected exosomes. (F) TEM for the appearance of transfected exosomes. Bar = 200 nm. ****P-value < 0.0001.