Fig. 4.

Effect of RA on constitutive activation of STAT3 and its relative proteins in the AOM/DSS-induced CAC in vivo mouse model. (A) The manifestation and translocation of pSTAT3 (Tyr705) in colon tissues were identified using IHC. Immunoreactivity of pSTAT3 in AOM/DSS-induced mice was assessed. (B) Nuclear (N) protein and total protein were extracted from the colon tissues of AOM/DSS-induced CAC mice. The phosphorylation of STAT3 at Tyr705 and Ser727 and the nuclear translocation of pSTAT3 (Try705) were estimated by western blotting in triplicate. (C) The expression of STAT3-relative proteins was confirmed by western blotting in triplicate. The relative ratio level was determined by densitometric analysis and normalized to internal controls. Numerical values are presented as means ± standard deviations (n = 8); ^###P < 0.001 compared with the control group; *P < 0.05, ^⁎⁎⁎P < 0.001 compared with the AOM/DSS-induced CAC group; significances between treated groups were determined by analysis of variance and Dunnett's post hoc test.