Fig. 5.

Effect of RA on TLR4 in the AOM/DSS-induced CAC in vivo model and molecular docking for the binding of RA to TLR4. (A) The expression of TLR4 in colon tissues of AOM/DSS-induced mice was identified using IHC. Immunoreactivity of TLR4 was assessed based on stained slides. (B) The expression of TLR4 in AOM/DSS-induced CAC mice colon tissue was estimated by western blotting in triplicate. Values are the means ± standard deviations (n = 8); ^##P < 0.01, ^###P < 0.001 vs the control group; *P < 0.05, ^⁎⁎P < 0.01, ^⁎⁎⁎P < 0.001 vs the AOM/DSS-induced CAC group; significances between treated groups were determined using an analysis of variance and Dunnett's post hoc test. (C) Molecular docking simulation was conducted to determine whether RA binds to TLR4 using the AutoDock Vina program.