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. 2021 May 24;12:665014. doi: 10.3389/fpls.2021.665014

Figure 3.

Figure 3

ag-circRBCS regulates RBCS gene expression. (A) Flowchart for artificial synthesis of ag-circRBCS and transported via layered double hydroxide lactate nanosheets (LDH-lactate-NS) in vitro. (B) The identification of synthesis ag-circRBCS via PCR. The convergent (con) and divergent (di) primers were used for analysis. (C) Analysis of the adsorption efficiency of LDH-lactate-NS in vitro. (D) Relative expression level of RBCS genes under different treatments. Numbers in (B,C) indicate the expected RNA length. (E) Analysis of the RBCS protein content via ELISA. M, marker; linear, synthesized single strand RNA before circulation; LDH, layered double hydroxide lactate nanosheets. Actin was used as an internal control. Data are expressed as mean ± standard deviation from three independent experiments. All the data were analyzed for significant differences using ANOVA with Duncan's test. Different lowercase letters represent statistical significance of p < 0.05 (n = 3).