FIGURE 5.

Adipose‐specific ATGL deletion rescues against fatty liver development post‐burn injury: Twelve‐week‐old ATGL floxed and knockout mice were treated with 30%TBSA injury and monitored daily for 7 days. (A) Oil Red O staining (zoom in and zoom out) for visualization of fat droplets in liver sections. (B) Tri‐acylglycerol (TAG) content in liver samples normalized to tissue weight. (C) Aspartate aminotransferase (AST) levels in serum samples. (D) Alanine aminotransferase (ALT) levels in serum samples. (E) Western blot analysis for the expression of targeted proteins (p‐HSL ser 563, HSL, ATGL, and actin) in the liver. (F) Western blot analysis and quantification of the expression of targeted proteins (Fas, Scd1, and gapdh) in the liver. (G) Quantitative PCR analysis of multiple genes in the liver. The results displayed are the average and SEM analyzed in the specified number of mice samples. Statistical significance was assessed using two‐way ANOVA as appropriate