FIGURE 4.

MLT inhibits lipid accumulation and PCa cell activity via CES1 expression. C4‐2 and 22RV1 cells with stable knockout of CES1 expression were generated and treated with DMSO or 1 mM MLT for 48 h. (A) Western blotting was performed to detect the CES1 expression in the groups. Densitometry and statistical analysis. Representative images are shown. (B and C) Cell viability and invasion under all conditions were determined using CCK‐8 and Transwell assays, respectively. (D) Knockout of CES1 abolished the inhibitory effect of melatonin on lipid accumulation. Representative images of BODIPY staining under all conditions. Quantification of the corrected total cell fluorescence (CTCF). (E) TG and T‐CHO contents were measured in all groups of cells. The graphs represent the means ± SEM. (F) Western blotting was performed to detect the expression of PPARa and ER stress marker genes in all groups. Densitometry and statistical analysis. Representative images are shown. A representative image of three independent experiments is shown. *p <  0.05, **p <  0.01, and ***p < 0.001. Abbreviation: ns, no significance