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. 2021 May 24;12:619069. doi: 10.3389/fimmu.2021.619069

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Copyright © 2021 Wurzer, Filali, Hoffmann, Krecke, Biolato, Mastio, De Wilde, François, Largeot, Berchem, Paggetti, Moussay and Thomas

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Primary human NK cells can invoke the actin response in MEC-1 and JVM-3 cells. (A) Cytotoxicity assay with primary NK cells isolated from four healthy human donors and JVM-3 and MEC-1 CLL target cells at 1:1 and 5:1 E:T ratios. Target cells and primary NK cells were co-cultured for 4 hrs before analysis of NK cell-specific lysis by flow cytometry. (B) Quantitative analysis of primary NK-CLL conjugates with (black, AR⁺) and without (grey, AR^-) actin response by imaging flow cytometry. For each donors a minimum of 200 conjugates were analyzed. ** denotes p < 0.001 (C) Representative confocal microscopy pictures of primary NK cells (CMRA, red) in conjugation with MEC-1 (left) and JVM-3 (right) cells with or without an actin response. Bars: 10 µm.