FIGURE 3.

Immunofluorescence and immunohistochemical staining analyses of the binding specificity of Z_HPV16E6 affibodies to HPV16 E6. (A) Representative images showing TC-1, CaSki cells (HPV16 positive), HeLa229 (HPV18 positive), and C666-1 cells (HPV negative) stained with Z_HPV16E61115, Z_HPV16E61171, and Z_HPV16E61235. The Z_WT affibody was used as a negative control. The affibody molecule stain is shown in green, while the nuclear stain (PI) is shown in red; magnification at ×400. (B) Representative image of HPV16-positive cervical cancer sections and HPV-negative normal human sections by hematoxylin and eosin (HE) staining and immunohistochemistry (IHC) staining with Z_HPV16E6 affibodies. Sections from HPV16-positive cervical cancer sections (upper panel) and HPV-negative normal human sections (lower panel) were labeled with Z_HPV16E6 affibodies. Polyclonal HPV16 E6 antibody was used as a positive control. Z_WT and PBS were used as negative controls. Magnification at ×400.