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. 2021 May 1;25:11–24. doi: 10.1016/j.omtn.2021.04.022

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© 2021 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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GAS6-AS1 functioned as a tumor suppressor in LUAD

(A) GAS6-AS1 was effectively upregulated in A549 and PC9 cells after transfection of overexpression plasmid of GAS6-AS1. (B and C) CCK8 assays were used to detect cell proliferation of A549 cells (B) and PC9 cells (C) after ectopic expression of GAS6-AS1. (D and E) EdU assays were performed in A549 (D) and PC9 cells (E) after ectopic expression of GAS6-AS1. (F and G) Transwell (F) and Matrigel (G) assays were used to test the migration and invasion ability of LUAD cells after ectopic expression of GAS6-AS1, respectively. (H) Images of xenograft tumors derived from nude mice bearing A549 cells of different groups. (I) Tumor volume was measured every week after injection. (J) Tumor weight was measured after resection of xenograft tumors.