FIGURE 5.

Running endurance evaluation following bleomycin (BLM) induction in rat chronic model of lung fibrosis. a) Animal stratification chart following treadmill training and BLM induction. b) Running distance before and after BLM induction, prior to ND-L02-s0201 treatment initiation. Last minute running distance recorded on Days 3, 7, and 16 for animals with or without BLM inductions (as indicated by triangles on Days 0–6 and Day 14, same regimen as figure 1a). c) Randomisation of BLM-treated animals into Vehicle and ND-L02-s0201 treatment group (n=10 each), based on Day 16 treadmill running distance. d) Average running distance per minute of total 7-min test duration at a speed of 60 cm·s⁻¹ during siRNA treatment period (Days 18–49). ND-L02-s0201 2 mg·kg⁻¹ treatment was delivered weekly starting on Day 18 and continued for 4 weeks. Treadmill tests were conducted three times per week for animals not treated with BLM (Sham), treated with BLM (Vehicle), and treated with BLM and ND-L02-s0201 (ND-L02-s0201). e) Final treadmill running test result on Day 49. *: p<0.05; ****: p<0.0001.