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. 2021 May 24;12:679152. doi: 10.3389/fphys.2021.679152

FIGURE 5.

FIGURE 5

High glucose concentration deteriorates myoblast differentiation. (A) Representative immunofluorescent images of the C2C12, H26M, and H35F myoblasts differentiated in DIM-NG or -HG. Scale bar, 200 μm. Ratio of MHC+ cells and multinuclear myotubes were quantified. *p < 0.05, **p < 0.01 vs. NG (Student’s t-test); n = 4–6. (B) qPCR results of myogenic gene expression in the C2C12 cells differentiated in DIM for 1 day. Mean value of NG group was set to 1.0 for each gene. *p < 0.05, **p < 0.01 vs. NG (Student’s t-test); n = 3.