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. 2021 May 24;12:679152. doi: 10.3389/fphys.2021.679152

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Copyright © 2021 Nakamura, Yonekura, Shimosato and Takaya.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Palmitic acid (PA) deteriorates myoblast differentiation. (A) Representative immunofluorescent images of the H26M and H35F myoblasts differentiated in DIM-NG with PA for 2 days. Scale bar, 200 μm. Ratio of MHC⁺ cells and multinuclear myotubes were quantified. **p < 0.01 vs. control (Student’s t-test); n = 4–6. (B,C) qPCR results of gene expression in the H35M myoblasts differentiated in DIM-NG with palmitic acid for 2 days. Mean value of control group was set to 1.0 for each gene; n = 1.