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. 2021 May 24;12:661379. doi: 10.3389/fimmu.2021.661379

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Copyright © 2021 Ferrara, Del Rosario, da Costa, Kinsley, Scott, Fereidouni, Thompson, Kellam, Gilbert, Carnell and Temperton

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Influenza B pseudotypes are expressed in different cell lines. (A) Titers of influenza B pseudotypes expressing firefly luciferase in HEK293T/17, MDCK, and A549 cells are shown in RLU/mL as the mean and standard deviation of 4 replicates. A ΔHA control representing non-HA pseudotyped virus was included for comparisons. No significant difference between PV titers and ΔHA is indicated by brackets (n.s. p>0.05). (B) Influenza B lentiviral pseudotype B/Florida/4/2006 containing an emerald GFP reporter is shown to successfully transduce HEK293T/17, MDCK and A549 cells. Scale bar = 6 µm.